|
Technical
requirements and procedures |
|
Form and quantity
The NBAIM prefers to receive micro-organisms submitted
for deposit as lyophilized preparations. Where it is undesirable
or impossible to supply lyophilized preparations, active
cultures growing in or on a suitable nutrient medium are
acceptable. The minimum number of replicates that must
be supplied by the depositor when making his deposit is
as follows: |
| |
| Fungi |
12
lyophilized ampoules (preferably), or 2 agar slants
|
| Yeasts |
12
lyophilized ampoules plus 1 agar slant (preferably),
or 2 agar slants |
| Bacteria |
12
lyophilized ampoules plus 1 agar slant (preferably),
or 2 agar slants
|
|
| |
In
cases where the depositor is unable to supply lyophilized
preparations, the NBAIM will prepare lyophilized cultures
at the time of deposit from the material supplied by the
depositor.
|
| |
Time
required for viability testing |
|
The average length of time required for testing viability
is 2 weeks for fungi, bacteria, actynomycetes and yeasts,
but occasionally viability testing may take longer, particularly
if unusual antibiotics or other additives are necessary
in the medium.
|
|
|
Checking
and renewal of stocks by the depositor |
| |
The NBAIM routinely asks the depositor to check the authenticity
of the deposited preparations after subculturing.
New batches of cultures are prepared whenever it is necessary
to renew diminishing stocks.
Whichever method is used for preparing batches of samples
for distribution, the NBAIM always keeps a portion of
the original material supplied by the depositor.
|
